HCG (Human Chorionic Gonadotropin) – User Manual & Dosage Protocol Guide

HCG (Human Chorionic Gonadotropin) is a glycoprotein hormone that mimics luteinizing hormone (LH) activity by stimulating LH receptors. It is FDA-approved for specific medical indications under physician supervision.

HCG is commonly studied in:

• Gonadal function research
• Testosterone production models
• Fertility and reproductive endocrinology investigations
• Hypogonadotropic hypogonadism protocols
• Hormonal axis restoration models

Disclaimer: HCG is a prescription medication. Use must be supervised by a licensed medical professional. The following information reflects labeled and commonly referenced clinical protocols.

Lyophilized Powder

Freeze-dried HCG intended for reconstitution prior to use.

Pre‑mixed Solution

A ready‑to‑use injectable format prepared by the manufacturer.

Typical Vial Strengths

Common vial sizes include 5,000 IU and 10,000 IU, with other strengths available depending on the manufacturer.

HCG functions by:

• Binding to LH receptors in the testes (males) or ovaries (females)
• Stimulating testosterone production in males
• Triggering ovulation in females
• Supporting spermatogenesis in hypogonadal males
• Maintaining corpus luteum function in fertility protocols

Male Hypogonadotropic Hypogonadism (Clinical Use)

Typical protocols use 500–1,000 IU administered subcutaneously (SC) or intramuscularly (IM), usually 2–3 times per week.

Fertility Protocol (Male)

Common dosing ranges from 1,000–3,000 IU, administered SC or IM, typically 2–3 times weekly as part of fertility-focused treatment plans.

Ovulation Induction (Female)

A single IM dose of 5,000–10,000 IU may be used as directed to support ovulation induction.

Testosterone Replacement Therapy (Adjunct Use)

In some TRT protocols, 250–500 IU may be administered subcutaneously, typically 2–3 times weekly.

Important

Dosage must be prescribed and individualized by a licensed healthcare provider based on laboratory values such as LH, FSH, testosterone, and estradiol, along with proper medical supervision.

Initiation

A 2–4 week phase used to monitor initial hormonal response to treatment.

Active Treatment

An ongoing phase focused on maintaining testosterone levels or fertility support as directed.

Adjustment Phase

Dosing or treatment plans may be modified as needed based on laboratory monitoring and clinical response.

Supplies

• Sterile bacteriostatic water (if not included)
• Sterile syringe
• Alcohol swabs

Example Reconstitution

If using 5,000 IU vial:

• Add 5 mL bacteriostatic water
  → Concentration = 1,000 IU per mL

Gently swirl to dissolve (do not shake).
Refrigerate after reconstitution.

Subcutaneous Injection (Common Method)

• Abdomen preferred
• Clean site with alcohol
• Inject slowly
• Rotate sites

Intramuscular Injection

• Gluteal muscle
• Deltoid (smaller volumes)

Follow sterile technique.

Lyophilized Powder

Store refrigerated or at room temperature as directed by the manufacturer, with a shelf life up to the expiration date.

Reconstituted Solution

Keep refrigerated at 2–8°C and use within 30–60 days, depending on the diluent.

Protection Guidelines

Protect from heat, light, and contamination. Do not freeze unless specifically instructed.

HCG may influence:

• Estradiol levels
• Testosterone levels
• Fluid retention
• Mood
• Gynecomastia risk (via estrogen conversion)

Common Monitoring Labs

• Total Testosterone
• Free Testosterone
• Estradiol (E2)
• LH/FSH
• Hematocrit

Possible Side Effects

• Injection-site irritation
• Water retention
• Elevated estradiol
• Mood fluctuations
• Headache

HCG should only be used under medical supervision.

Compound: Human Chorionic Gonadotropin (HCG)
Category: Gonadotropin Hormone
Delivery: SC or IM injection
Typical Clinical Range: 250–3,000 IU depending on indication
Monitoring: Testosterone, Estradiol, LH/FSH
Storage: Refrigerate after reconstitution